Description
A gram of NAD+ is a working stock, not a sample. This fill suits labs that use the coenzyme as a routine reagent in dehydrogenase, sirtuin or PARP assays.
NAD+ 1000 mg for assay-scale work
Because NAD+ is used at millimolar concentrations in many enzyme assays, consumption adds up faster than it does for signalling peptides used at nanomolar levels. One large vial avoids mixing lots partway through a study, and keeps the cost per milligram at its lowest.
The reduced form, NADH, has a distinct absorbance peak at 340 nm that NAD+ lacks, so spectrophotometry offers a quick check that material has not been reduced during handling. For longer-term storage, keep the solid frozen and dry and make up solutions fresh or in frozen single-use portions.
Compound identity and specification
| Compound | NAD+ |
|---|---|
| Sequence | Not a peptide: nicotinamide adenine dinucleotide, a dinucleotide of nicotinamide mononucleotide and adenosine monophosphate |
| Molecular formula | C21H27N7O14P2 |
| Molecular mass | 663.4 g/mol |
| CAS registry number | 53-84-9 |
| PubChem | CID 5892 |
| Vial content | 1000 mg |
| Physical form | Lyophilised powder in a sealed vial |
| Analysis | Third-party HPLC and purity testing to a 99%+ specification; certificates are posted on our COAs page |
| Supplied for | Laboratory research use only |
Questions about NAD+ 1000mg
How can I check NAD+ has not been reduced?
NADH absorbs at 340 nm; NAD+ does not. A clean 260/340 profile is consistent with the oxidised form.
Does NAD+ need to be frozen?
Freezing the dry solid is the safest long-term option. Protect it from moisture above all.
Do quantity breaks apply to NAD+?
Yes, on the same ladder as the peptides: 5% off from two vials, 35% off from ten.
Research literature
Peer-reviewed studies involving this compound and its class. All findings listed are from in vitro, cell culture or animal models and are provided for scientific reference only. This product is supplied for laboratory research and is not approved for human consumption, clinical, or veterinary use.
- Canto C, et al. Cell Metab. 2012;15(6):838-847. In mammalian cells and mouse skeletal muscle and brown adipose tissue, measured tissue NAD+ levels and SIRT1/SIRT3 target deacetylation. Source · PMID 22682224
- Gomes AP, et al. Cell. 2013;155(7):1624-1638. In aged mouse skeletal muscle, measured nuclear NAD+ decline, normoxic HIF-1alpha accumulation and loss of mtDNA-encoded OXPHOS subunits. Source · PMID 24360282
- Mills KF, et al. Cell Metab. 2016;24(6):795-806. In C57BL/6N mice over 12 months, measured tissue NAD+ synthesis, cellular energetics, insulin sensitivity and skeletal-muscle mitochondrial oxidative capacity. Source · PMID 28068222
Literature notes: These studies concern NAD+ precursors (nicotinamide riboside and nicotinamide mononucleotide) rather than NAD+ administered directly.
Certificates for our compounds are posted on the certificates of analysis page and matched to vials by cap and crimp colour. If the one for this product is not up yet, contact the order desk.
Related research compounds
Browse more compounds in Mitochondrial & Cellular Compounds, or view the full research peptide catalogue.
Supplied for laboratory research use only. Not for human or veterinary use.
