BPC-157 TB-500 Canada: Blend Sizes, Identity Data and Lot Checks
For labs that always run BPC-157 and TB-500 side by side, a premixed vial removes a weighing step and halves the number of lines in the inventory. It also changes what the certificate has to show and how a QC team should check it. This guide covers BPC-157 TB-500 in Canada from a volume buyer’s point of view: what each blend size contains, why the two components are unrelated molecules, what makes BPC-157 in particular sensitive to chromatographic method, and how to decide between the blend and separate vials for a larger order.
What the two blend sizes contain
Both blends use an equal split by mass, freeze-dried together in one vial:
| Vial | BPC-157 | TB-500 | Total peptide |
|---|---|---|---|
| 10 mg blend | 5 mg | 5 mg | 10 mg |
| 20 mg blend | 10 mg | 10 mg | 20 mg |
An equal split by weight is not an equal split by molecule count. TB-500 is the smaller molecule, so 5 mg of it is about 5.6 micromoles, against about 3.5 micromoles for 5 mg of BPC-157. Each vial therefore holds roughly 1.6 TB-500 molecules for every BPC-157 molecule. If your in-vitro design compares the blend with single-compound controls, match on moles rather than milligrams.
Identity data for each component
| BPC-157 | TB-500 | |
|---|---|---|
| Sequence | GEPPPGKPADDAGLV (15 residues) | Ac-LKKTETQ (7 residues, N-acetylated) |
| Formula | C62H98N16O22 | C38H68N10O14 |
| Average mass | 1419.5 g/mol | 889.0 g/mol |
| Main ESI ions (approx.) | m/z 1419.7 (1+), 710.4 (2+) | m/z 889.5 (1+), 445.3 (2+) |
| CAS | 137525-51-0 | 885340-08-9 |
The two share no sequence and no structural family. They are combined for convenience in research programmes, not because one is a variant of the other.
TB-500 and thymosin beta-4 are different materials
Catalogues and forum posts often use these names as if they were interchangeable. They are not. Thymosin beta-4 is a 43-residue protein. TB-500, as supplied here, is an acetylated seven-residue peptide matching the 17 to 23 stretch of that protein. Its mass is a small fraction of the full protein’s, so a certificate for one can never stand in for the other.
This matters when you read the literature, too. A study of thymosin beta-4 is not automatically a study of the fragment. Keep the two as separate entries in your inventory and your reference files.
BPC-157’s proline cluster and why method matters
BPC-157 carries prolines at positions 3, 4, 5 and 8. Runs of proline are known to be difficult in solid-phase synthesis, and incomplete couplings there produce deletion impurities: chains identical to the target except for one missing proline, about 97 Da lighter. Because the rest of the chain is unchanged, such a deletion elutes very close to the main peak on reversed-phase HPLC.
That makes this compound sensitive to how the chromatography is run. A fast, steep gradient can fold a deletion peak into the main peak and report a higher purity. A shallower gradient that separates them reports a lower, more honest figure for the same material. When comparing certificates between lots or suppliers, check the gradient and column as well as the number. Our guide to reading a peptide chromatogram covers what to look for.
Reading a two-component report
A blend report should show two main peaks, one per component, each identified by retention against a standard and by mass. A single peak with one purity figure does not tell you how pure either peptide is.
Two further details are specific to this pair:
- Both are low-UV analytes. Neither sequence contains tryptophan, tyrosine or phenylalanine, so neither absorbs usefully near 280 nm. Detection relies on the 210 to 220 nm region, and a dual-wavelength identity check is not available.
- Areas are not amounts. Response at low wavelength depends mainly on the number of peptide bonds, and BPC-157 has roughly twice as many as TB-500. Equal milligrams will not give equal areas, and converting areas to milligrams needs a reference standard for each component.
Identity by mass and purity by HPLC also say nothing about sterility, endotoxin or residual solvents unless those assays are listed separately.
Blend or separate vials for BPC-157 TB-500 in Canada
The deciding question is whether the ratio in your work is fixed. If every experiment uses the two at a one-to-one mass split, the blend saves time and record keeping, and the 20 mg size halves the number of vials to log and open compared with the 10 mg size. If you need to vary the ratio, run either compound alone, or keep separate controls, single vials are the only option: BPC-157 5 mg or 10 mg, and TB-500 5 mg or 10 mg.
Many labs end up buying both. Our pricing tiers are mix-and-match, so blend vials and single-compound vials in one cart all count together toward the break. The full range sits under BPC-157 and TB-500 peptides.
Logging a blend order on arrival
- Separate blend vials from single-compound vials and check each label against the order.
- Sort vials by cap and crimp colour, which is how each vial is matched to its certificate.
- Find the corresponding document on our COA listings page if it has been posted; certificates are published for some products, and support can tell you what is on file for the rest.
- Record lot, vial count, date and storage location, and transfer everything to a cold, dark shelf before the end of the day.
Our range is third-party tested for purity by HPLC.
BPC-157, TB-500 and their blends are research chemicals for in-vitro laboratory use only. They are not for use in people or animals and are not approved by Health Canada as drugs.
Ordering both compounds for a larger study? The BPC-157 vs TB-500 bulk comparison works out cost per mg at every volume tier.
